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Mechanism of Uncoating of Influenza B Virus in MDCK Cells: Action of Chloroquine

Hydroxychloroquine Chloroquine Influenza Flu Infection Vaccination

“Exposure of influenza B virus-infected MDCK cells to chloroquine at the time of infection resulted in significant inhibition of infection.”

“It is likely that chloroquine prevents the uncoating of influenza B virus by increasing the lysosomal pH above the critical value required for inducing fusion between the virus envelope and the lysosomal membrane.”

Hydroxychloroquine Chloroquine Influenza Flu Infection Vaccination

“The normal pH inside the lysosomes was around 5-6. This value was within the pH range optimal for the haemolysis reaction by influenza B virus. When chloroquine was added to the culture, the lysosomal pH rapidly increased to 6-5, which is above the critical value required for haemolysis by influenza B virus.”

“These findings indicate that extracellular chloroquine is essential for the maintenance of elevated pH inside the lysosomes.”

Hydroxychloroquine Chloroquine Influenza Flu Infection Vaccination

“These results suggest that chloroquine inhibits infection by acting
at a replicative step preceding primary transcription of the virus genome. Similar conclusions have also been reported for the effect of another lipophilic amine, amantadine, on influenza A viral replication.”

Hydroxychloroquine Chloroquine Influenza Flu Infection Vaccination

Actinomycin D completely inhibited the growth of influenza B virus in MDCK cells when added within 2 h after infection, as observed with influenza A virus.”

Chloroquine inhibits infection by acting at a step preceding the primary transcription of the influenza B virus genome”Hydroxychloroquine Chloroquine Influenza Flu Infection Vaccination

“Further experiments were carried out to confirm that elevation of the lysosomal pH by chloroquine is responsible for its inhibition of infection.

https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-64-5-1149

 

By Paul

PAUL MUADDIB is a journalist, investigator and QAnon with a focus on uncovering the truth.

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